Selected Publications
2025
Tröster V, Wong RP, Börgel A, Cakilkaya B, Renz C, Möckel MM, Marinho J, Reinberg T, Furler S, Schaefer JV, Plückthun A, Wolf E, Eifler-Olivi K, Ulrich HD (2025). Custom affinity probes reveal chromatin SUMOylation in budding yeast. Cell Reports Vol. 44 Issue 3; doi: 10.1016/j.celrep.2025.115353
2024
Chawla S, O'Neill J, Knight MI, He Y, Wang L, Maronde E, Rodríguez SG, van Ooijen G, Garbarino-Pico E, Wolf E, Dkhissi-Benyahya O, Nikhat A, Chakrabarti S, Youngstedt SD, Zi-Ching Mak N, Provencio I, Oster H, Goel N, Caba M, Oosthuizen M, Duffield GE, Chabot C, Davis SJ. Timely Questions Emerging in Chronobiology: The Circadian Clock Keeps on Ticking. J Circadian Rhythms. 2024 Apr 2;22:2. doi: 10.5334/jcr.237.
Prorok P, Wolf E, Cardoso MC (2024). Timeless-Tipin interactions with MCM and RPA mediate DNA replication stress response. Frontiers in Cell and Developmental Biology Vol. 12; doi: 10.3389/fcell.2024.1346534
Behrmann, E., Wolf, E. Wie erkennt der innere Monatskalender von Tieren die richtige Mondphase?. Biospektrum 30, 766–768 (2024). https://doi.org/10.1007/s12268-024-2337-0
2023
Vu HH*, Behrmann H*, Hanić M, Jeyasankar G, Krishnan S, Dannecker D, Hammer C, Gunkel M, Solov’yov IA, Wolf E# and Behrmann E#. (2023) A marine cryptochrome with an inverse photo-oligomerization mechanism. Nat Commun 14, 6918. doi: 10.1038/s41467-023-42708-2
Mat A, Vu HH, Wolf E, Tessmar-Raible K. (2023) All light, everywhere? Photoreceptors at non-conventional sites. Physiology (Bethesda). 2023 Oct 31. doi: 10.1152/physiol.00017.2023
Giesecke A, Johnstone PS, Lamaze A, Landskron J, Atay E, Chen KF, Wolf E, Top D, Stanewsky R. (2023) A novel period mutation implicating nuclear export in temperature compensation of the Drosophila circadian clock. Curr Biol. 33(2):336-350.e5. doi: 10.1016/j.cub.2022.12.011
2022
Poehn B*, Krishnan S*, Zurl M, Coric A, Rokvic D, Häfker NS, Jaenicke E, Arboleda E, Orel L, Raible F, Wolf E # and Tessmar-Raible K # (2022). A Cryptochrome adopts distinct moon- and sunlight states and functions as sun- versus moonlight interpreter in monthly oscillator entrainment. Nat Commun 13: 5220; doi: 10.1038/s41467-022-32562-z
Zurl M, Poehn B, Rieger D, Krishnan S, Rokvic D , Rajan VBV, Gerrard E , Schlichting M, Orel L, Lucas RJ, Wolf E, Helfrich-Förster C, Raible F and Tessmar-Raible K (2022) Two light sensors decode moonlight versus sunlight to adjust a plastic circadian/circalunidian clock to moon phase. Proc Natl Acad Sci USA, 119(22):e2115725119, doi: 10.1073/pnas.2115725119
Daiber A, Frenis K, Kuntic M, Li H, Wolf E, Kilgallen AB, Lecour S, Van Laake LW, Schulz R, Hahad O, Münzel T (2022) Redox regulatory changes of circadian rhythm by the environmental risk factors traffic noise and air pollution. Antioxid Redox Signal. 37(10-12):679-703. doi: 10.1089/ars.2021.0272
2020
Junglas B, Orru R, Axt A, Siebenaller C, Steinchen W, Heidrich J, Hellmich UA, Hellmann N, Wolf E, Weber SAL and Schneider D (2020) IM30 IDPs form a membrane-protective carpet upon super-complex disassembly. Commun Biol, 3:595. doi: 10.1038/s42003-020-01314-4
Wolf E# and Kramer A# (2020) Circadian regulation: from molecules to physiology. J Mol Biol, 432:3423-3425. doi: 10.1016/j.jmb.2020.05.004
2019
Li H*, Kilgallen AB*, Münzel T, Wolf E, Lecour S, Schulz R, Daiber A# and Van Laake LW# (2019) Influence of mental stress and environmental toxins on circadian clocks - implications for redox regulation of the heart and cardioprotection. Br J Pharmacol, 177: 5393–5412. doi: 10.1111/bph.14949
Garg A, Orru R, Ye W, Distler U, Chojnacki JE, Köhn M, Tenzer S, Sönnichsen C and Wolf E (2019) Structural and mechanistic insights into the interaction of the circadian transcription factor BMAL1 with the KIX domain of the CREB-binding protein. J Biol Chem, 294:16604–16619, doi: 10.1074/jbc.RA119.009845.
2015
Merbitz-Zahradnik T and Wolf E (2015) How is the inner circadian clock controlled by interactive clock proteins? Structural analysis of clock proteins elucidates their physiological role. FEBS Letter, 589: 1516-1529. doi: 10.1016/j.febslet.2015.05.024.
Merbitz-Zahradnik T and Wolf E (2015) Neue Einsichten in die Funktionsweise der inneren Uhr. Biospektrum 05/2015, S. 493-495.
2014
Witosch J, Wolf E# and Mizuno N# (2014) Architecture and ssDNA interaction of the Timeless-Tipin-RPA complex. Nucleic Acids Research 2014 Nov 10;42(20):12912-12927. doi: 10.1093/nar/gku960
Schmalen I, Reischl S, Wallach T, Klemz R, Grudziecki A, Prabu JR, Benda C, Kramer A# and Wolf E# (2014) Interaction of circadian clock proteins CRY1 and PER2 is modulated by zinc binding and disulfide bond formation. Cell, 157:1203–1215, doi: 10.1016/j.cell.2014.03.057.
2013
Czarna A, Berndt A, Singh HR, Grudziecki A, Ladurner AG, Timinszky G, Kramer A and Wolf E (2013) Structures of Drosophila cryptochrome and mouse cryptochrome1 provide insight into circadian function. Cell, 153:1394–1405, doi: 10.1016/j.cell.2013.05.011
Makbul C, Constantinescu Aruxandei D, Hofmann E, Schwarz D, Wolf E and Herrmann C (2013) Structural and thermodynamic characterization of Nore1-SARAH: a small, helical module important in signal transduction networks. Biochemistry, 12;52(6):1045-54. doi: 10.1021/bi3014642
2012
Kucera N, Schmalen I, Hennig S, Ollinger R, Strauss HM, Grudziecki A, Wieczorek C, Kramer A and Wolf E (2012) Unwinding the differences of the mammalian PERIOD clock proteins from crystal structure to cellular function. Proc Natl Acad Sci USA, 109:3311–3316, doi: 10.1073/pnas.1113280109.
2011
Czarna A*, Breitkreuz H*, Mahrenholz CC, Arens J, Strauss HM and Wolf E (2011) Quantitative analyses of cryptochrome-mBMAL1 interactions: Mechanistic insights into the transcriptional regulation of the mammalian circadian clock. J Biol Chem, 286:22414–22425, doi: 10.1074/jbc.M111.244749.
Pawlowski N, Khaminets A, Hunn JP, Papic N, Schmidt A, Uthaiah RC, Lange R, Vopper G, Martens S, Wolf E and Howard J (2011) The activation mechanism of Irga6, an interferon-inducible GTPase contributing to mouse resistance against Toxoplasma gondii. BMC Biol. 28; 9(1): 7, doi: 10.1186/1741-7007-9-7
2009
Hennig S, Strauss HM, Vanselow K, Yildiz O, Schulze S, Arens J, Kramer A and Wolf E (2009) Structural and functional analyses of PAS domain interactions of the clock proteins Drosophila PERIOD and mouse PERIOD2. PLOS Biol, 7:e94. doi: 10.1371/journal.pbio.1000094.
Landskron J, Chen KF, Wolf E and Stanewsky R (2009) A role of the PERIOD:PERIOD Homodimer in the Drosophila circadian Clock. PLoS Biology 28;7(4):e3. doi: 10.1371/journal.pbio.1000003
Tyagi A, Penzkofer A, Batschauer A and Wolf E (2009) Fluorescence Behaviour of 5,10-Methenyl-tetrahydrofolate, 10-Formyltetrahydrofolate, 10-Formyldihydrofolate, and 10-Formylfolate in Aqueous Solution at pH 8. Chemical Physics 361 (1-2), 75-82
Tyagi A, Penzkofer A, Batschauer A and Wolf E (2009) Degradation of (6R,S)-5,10-Methenyltetra-hydrofolate in aqueous Solution at pH 8 and pH 2.5. Chemical Physics 358 (1-2), 132-136
2008
Hoang N, Schleicher E, Kacprzak S, Bouly JP, Picot M, Wu W, Berndt A, Wolf E, Bittl R and Ahmad M (2008) Human and Drosophila Cryptochromes are light activated by Flavin Photoreduction in living Cells. PLoS Biology 2008 Jul 1;6(7):e160, doi: 10.1371/journal.pbio.0060160.
Shirdel J, Zirak P, Penzkofer A, Breitkreuz H and Wolf E (2008) Fluorescence spectroscopic Characterization of the circadian blue-light Photoreceptor Cryptochrome from Drosophila melanogaster. Chemical Physics 28; 352(1): 35-47
2007
Berndt A, Kottke T, Breitkreuz H, Dvorsky R, Hennig S, Alexander M and Wolf E (2007) A novel photoreaction mechanism for the circadian blue light photoreceptor Drosophila cryptochrome. J Biol Chem, 282:13011–13021, doi: 10.1074/jbc.M608872200.
Schöning JC., Streitner C., Page DR., Hennig S., Uchida K., Wolf E., Furuya M., Staiger D. (2007) Autoregulation of the circadian slave oscillator component ATGRP7 and regulation of its target genes is impaired by a single RNA recognition motif point mutation. The Plant Journal 52(6):1119-30, doi: 10.1111/j.1365-313X.2007.03302.x
Penzkofer A., Shirdel J., Zirak P., Breitkreuz H. and Wolf E (2007) Protein Aggregation Studied by Forward Light Scattering and Light Transmission Analysis. Chemical Physics 342(1): 55-63
2005
Yildiz O, Doi M, Yujnovsky I, Cardone L, Berndt A, Hennig S, Schulze S, Urbanke C, Sassone-Corsi P and Wolf E (2005) Crystal structure and interactions of the PAS repeat region of the Drosophila clock protein PERIOD. Mol Cell, 17:69–82, doi: 10.1016/j.molcel.2004.11.022.
2004
Ghosh A, Uthaiah R, Howard J, Herrmann C and Wolf E (2004) Crystal Structure of IIGP1: A Paradigm for Interferon-Inducible p47 Resistance GTPases. Mol Cell 15(5):727-739. doi: 10.1016/j.molcel.2004.07.017
Martens S, Sabel K, Lange R, Uthaiah R, Wolf E and Howard J.C. (2004) Mechanisms Regulating the Positioning of Mouse p47 Resistance GTPases LRG-47 and IIGP1 on Cellular Membranes: Retargeting to Plasma Membrane Induced by Phagocytosis. The Journal of Immunology 173(4): 2594-2606. doi: 10.4049/jimmunol.173.4.2594
Kühnel K, Jarchau T, Wolf E, Schlichting I, Walter U, Wittinghofer A and Strelkov SV (2004) The VASP tetramerization domain is a right-handed coiled coil based on a 15-residue repeat. Proc Natl Acad Sci USA 101(49):17027-32. doi: 10.1073/pnas.040306910
2003
Rehmann H *, Prakash B *, Wolf E *, Rueppel A, de Rooij J, Bos JL and Wittinghofer A (2003) Crystal Structure of the Regulatory Domain of Epac2 suggests a conserved mode of cAMP regulation. Nat Struct Biol 10(1):26-32. doi: 10.1038/nsb878
2002
Wolf E, DeAngelis J, Khalil E.M, Cole PA and Burley SK (2002). X-ray crystallographic studies of Serotonin-N-acetyltransferase catalysis and inhibition. J Mol Biol 317(2):215-24, doi: 10.1006/jmbi.2001.5371
2001
Wurtele M, Wolf E, Pederson KJ, Buchwald G, Ahmadian MR, Barbieri JT and Wittinghofer A (2001) How the Pseudomonas aeruginosa ExoS toxin downregulates Rac. Nat Struct Biol 8(1):23-6, doi: 10.1038/83007
Wurtele M, Renault L, Barbieri JT, Wittinghofer A and Wolf E (2001) Structure of the ExoS GTPase activating domain. FEBS Lett 491(1-2):26-9, doi: 10.1016/s0014-5793(01)02105-6.
1998
Wolf E, Vassilev A, Makino Y, Sali A, Nakatani Y and Burley SK (1998) Crystal structure of a GCN5-related N-acetyltransferase: Serratia marcescens Aminoglycoside 3-N-acetyltransferase. Cell 1998 94(4):439-49, doi: 10.1016/s0092-8674(00)81585-8
1995
Wolf E, Brukner I and Suck D (1995) Mutational analysis of DNase I-DNA interactions: Design, expression and characterization of a DNaseI loop-insertion mutant with altered sequence selectivity. Protein Engineering 8(3):283-91, doi: 10.1093/protein/8.3.283